ELISA
The Enzyme-Linked Immunosorbent Assay (ELISA) is a well-established cost-effective plate-based analytical technique used for the detection and quantification of soluble substances such as proteins, peptides, antibodies, and hormones. The service covers the execution of standard commercial kits as well as the complete de novo development and validation of custom ELISAs tailored to specific analytical requirements. The workflow includes sample processing from a variety of biological matrices (e.g., serum, plasma, urine, cell culture supernatants, tissue homogenates) across multiple species, including murine models and large animals. We offer in-house antibody conjugation and biotinylation services and assay development. In addition, we provide custom development of non-commercially available antibodies or antisera, as well as recombinant production and sequence optimization of a wide range of antibody formats from customer-provided sequences using mammalian expression systems
Applications
| Application | Description |
|---|---|
| Pharmacokinetics (PK/PD) | Quantification of biotherapeutics and target engagement in preclinical species. |
| Antibody Titer Determination | Quantification of antigen-specific antibody titers (e.g., total IgG, IgG subclasses, IgM, or IgA) in serum or plasma to evaluate the magnitude of the humoral immune response induced by candidate vaccines. |
| Immunogenicity & ADA | Screening, confirmatory, and titration assays for Anti-Drug Antibodies. |
| Biomarker Analysis | Quantification of soluble targets across diverse biological matrices. |
Key Features
- Custom Assay Development: Tailored ELISA design, optimization, and validation for specific targets.
- In-house Antibody Production: Generation of custom antibodies using mammalian expression systems.
- Low Sample Volume Requirements: Reliable quantification from limited sample amounts.
- Cost-effective Solutions: Flexible assay formats with high analytical performance.
- Cross-species and matrix versatility: Validated ELISA workflows for multiple biological matrices across rodent, large-animal, and human samples.