ECLIA on the MSD platform
Electrochemiluminescence immunoassays (ECLIA) on the Meso Scale Discovery (MSD) platform combine ultra-sensitive detection, broad dynamic range, and multiplexing capabilities to deliver high-quality biomarker data from limited sample volumes. Using the MESO QuickPlex SQ 120MM platform, up to 10 analytes can be quantified simultaneously in a single well, enabling comprehensive profiling of inflammatory, immunological, and metabolic pathways.
Compared to conventional ELISA workflows, ECLIA technology provides enhanced sensitivity, wider quantification ranges, and increased analytical efficiency through multiplex analysis, maximizing the amount of information obtained from valuable preclinical samples.
Our services support pharmacokinetic assessment of biotherapeutics, biomarkers profiling, pharmacodynamic studies, and translational research across rodent, large-animal, and human matrices. Both commercial ECLIA panels and custom-developed assays can be implemented to meet specific project requirements.
Applications
| Application | Description |
|---|---|
| Multiplex Inflammatory & Metabolic Profiling (V-PLEX / U-PLEX) | High-throughput, ultra-sensitive multiplex analysis of inflammatory and metabolic biomarkers in mouse, rat, and human samples using validated V-PLEX panels or customizable U-PLEX assays, with up to 10 analytes measured simultaneously in a single well. |
| Pharmacokinetics (PK/PD) | Highly sensitive (low nanogram to picogram range) quantification of biotherapeutics and assessment of treatment-induced biomarker modulation. |
| Assay Transfer & Sensitivity Enhancement | Conversion and optimization of existing ELISA methods to ECLIA formats to improve sensitivity, dynamic range, and analytical performance while maintaining assay continuity. |
| Biomarker Discovery & Validation | Evaluation and validation of soluble biomarkers associated with efficacy, disease progression, target engagement, or safety. |
| Custom Assay Development | Design, optimization, and validation of tailored ECLIA assays for novel targets or specific biomarker panels not covered by commercial kits. |
Key Features
- Ultra-sensitive detection with broad dynamic range
- Multiplex analysis of up to 10 analytes per well
- High-throughput biomarker profiling
- Quantification of biologics and biotherapeutics
- Custom ECLIA assay development, including antibody SulfoTag coupling
- ELISA-to-ECLIA method conversion for enhanced sensitivity
- Compatibility with standard and complex biological matrices
- Suitable for rodent, large-animal, and human samples