Technology

Confocal Imaging

Our confocal imaging services support medium-throughput cellular imaging, phenotypic characterization, and longitudinal biological studies using the Agilent BioTek Cytation C10 Confocal Imaging Reader. Combining laser-based confocal imaging with automated image acquisition and quantitative analysis, the platform enables high-quality fluorescence and brightfield imaging for preclinical, translational, and cell biology research in multiwell plate formats.

Applications

ApplicationDescription
Phenotypic ScreeningQuantitative assessment of cellular morphology, viability, proliferation, and functional responses.
Live-Cell ImagingTime-lapse monitoring of cellular behavior, migration, growth, and treatment responses.
Immunofluorescence AnalysisVisualization and quantification of protein expression and intracellular localization.
3D Cell Model AnalysisPhenotypic analysis of engineered and primary 3D cell populations, including spheroids and organoids.
Cell Therapy ResearchCharacterization of engineered and primary cell populations using multiplex fluorescence imaging.
Longitudinal StudiesStandardized image acquisition and analysis across multiple study time points.
Inflammation & Toxicity ResearchAnalysis of cellular activation, structural integrity, and inflammatory or cytotoxic responses.
Translational StudiesIntegration of imaging endpoints into efficacy, biodistribution, and mechanism-of-action workflows.

Key Features

  • LDI-7 Laser Illuminator providing stable, high-intensity excitation for multiplex fluorescence imaging
  • Laser-scanning confocal imaging for improved optical sectioning and background reduction
  • Laser autofocus system ensuring accurate focus maintenance during automated imaging workflows
  • Multi-mode imaging capabilities including: Confocal fluorescence imaging; Widefield fluorescence imaging; Brightfield imaging; Phase contrast imaging.
  • Compatible with standard multiwell plate formats for medium-throughput screening applications
  • Automated image acquisition and analysis workflows
  • Quantitative single-cell and population-level measurements
  • High-resolution imaging with a range of objectives optimized for screening and detailed cellular analysis
  • Reproducible longitudinal image acquisition across complex study designs
  • Flexible assay development and image analysis support