Hematology & Hemostasis
We provide a robust portfolio of advanced in vitro and in vivo hematology assays, specifically engineered to accelerate breakthrough research in hemostasis, thrombosis, and bleeding disorders. Through standardized workflows, specialized murine models, and cutting-edge monitoring systems, we deliver high-precision characterization of treatment efficacy and pharmacokinetic profiles.
Specialized in vivo Bleeding Models & Efficacy Testing
Because hemostasis relies on complex physiological interactions, our in vivo portfolio features robust injury and bleeding models specifically designed to assess hemostatic recovery across a variety of coagulation-deficient strains.
Coagulation-Compromised Phenotypes
- Factor VIII (F8) knockout
- Immunoglobulin heavy chain µ (Ighm) and F8 knockout
- Factor IX (F9) knockout
- Plasminogen (Plg) knockout
Monitoring Strategies
| Title | Description |
|---|---|
| Validated Bleeding Challenge Models | Tail-clip, nail-clip, and saphenous vein cut assays to assess real-time clotting and vessel repair |
| Functional Activity Tracking | Longitudinal monitoring of running behavior and mobility via the automated REVOLYZER 3TS system (preclinics, Potsdam, Germany) |
| Clinical Assessments | Real-time scoring of active bleeds, hematomas, and localized muscle swelling |
| Health Assessment | Regular health evaluations including longitudinal body weight tracking, genetic phenotyping, and whole-blood analysis |
Modeling Secondary Complications: Hemophilic Arthropathy
The debilitating secondary joint complications of coagulation pathologies can be precisely evaluated using our established knee hemarthrosis model. We track joint disease progression and therapeutic protection through an integrated endpoint pipeline:
| Title | Description |
|---|---|
| in life Metrics | Real-time tracking of ambulatory behavior (joint pain/mobility) paired with precise caliper measurements of knee diameter to track inflammation |
| ex vivo Characterization | High-resolution Micro-CT (µCT) imaging for 3D bone architecture evaluation, alongside specialized joint histopathology to score synovitis and cartilage degradation |
Advanced Pharmacokinetics (PK)
To ensure a comprehensive evaluation of your therapeutic candidates, we offer complete pharmacokinetic (PK) analysis supported by advanced technical workflows:
| Title | Description |
|---|---|
| Flexible Administration Routes | Mastery of standard dosing modalities as well as advanced surgical methodologies, including continuous or targeted catheterization. |
| Fluid and Tissue Sampling | Reliable serial and endpoint harvesting of plasma, serum, whole blood, and organs |
| Holistic in vivo Mapping | Integration of PK data with imaging analysis for a multi-dimensional view from absorption to elimination |
Comprehensive in vitro Hematology & Bioanalytical Assays
To complement our in vivo studies, we offer a fully validated suite of in vitro assays. These services provide deep mechanistic insight, precise quantification of the administered test item, and profiling using human, murine, and multi-species plasma samples.
| Title | Description |
|---|---|
| Antigen Quantification | ELISA-based assays to precisely quantify FVIII and FIX antigen levels in the plasma of treated, factor-deficient mice |
| Functional Activity Assays | Chromogenic assays established for both human and mouse plasma to measure functional FVIII, FIX, and VWF activity |
| Coagulation Kinetics | Global chromogenic assessment of plasma kinetic profiles and thrombin generation capacity via the Thrombin Generation Assay (TGA) |
| Complete Blood Count | High-precision automated hematology using the Exigo H400, validated for murine and multi-species samples to profile RBC, WBC, PLT, HGB, and HCT |
| Blood Loss Quantification | Semi-quantitative or quantitative assays to measure total blood loss during in vivo bleeding challenges via hemoglobin concentration profiling. |
| Immune Profiling | Precision-gated flow cytometry to quantify absolute cell counts and frequency percentages of T, B, and NK cell populations in whole blood, spleen, or bone marrow samples |
| Cytokine Profiling | High-sensitivity multiplex assays (flow cytometry, ECLIA) for rapid screening of cytokines of interest |
Rely on our expertise to make informed Go/No-Go decisions for your development program.
Thanks to our experienced team we are always ready to establish new bleeding assays for you. Our team can support you in the best assay selection to establish your goals.